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KMID : 0903519980410020119
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1998 Volume.41 No. 2 p.119 ~ p.124
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Isolation and Identification of Fibrinolytic Bacteria from Korean Traditional Chungkookjang
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Abstract
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In this study, the bacteria which could hydrolyze the fibrin produced through the blood coagulation mechanism in the human body, were isolated from Chungkookjang. The KCK-7 strain was selected among the isolated bacteria as the best strain for fibrinolytic activity. It was spore forming and Gram positive. C_(15-0) anteisa fatty acid and C_(15-0) iso fatty acid were 40.85% and 19.47%, respectively as major component among its cellular fatty acid composition. It showed the similarity of 63.6%, compared with standard strain. It was thus identified to be Bacillus subtilis according to Bergey´s manual of systematic bacteriology and its fatty acid profiles af Gas chromatography. The optimum culture temperature and pH were 37℃ and 8 for the production of fibrinolytic enzyme by Bacillus subtilis KCK-7.
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